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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol 9 No 1 (2003): December 2003" : 11 Documents clear
ISOLATION AND CULTURE OF Celosia cristata L. CELL SUSPENSION PROTOPLASTS Retno Mastuti; Hiroshi Miyake; Takeshi Taniguchi; Yoji Takeoka
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/405

Abstract

Developmental competence of Celosia cristata L. cell suspension-derived protoplasts was investigated. The protoplasts were isolated from 3- to 9-d old cultures in enzyme solution containing 2% (w/v) Cellulase YC and 0.5% (w/v) Macerozyme R-10 which was dissolved in washing solution (0.4 M mannitol and 10 mM CaCl2) at pH 5.6 for 3 hours. The highest number of viable protoplasts was released from 5-d old culture of a homogenous cell suspension. Subsequently, three kinds of protoplast culture media were simultaneously examined with four kinds of concentration of gelling agent. Culturing the protoplasts on KM8p medium solidified with 1.2% agarose significantly enhanced plating efficiency as well as microcolony formation. Afterwards, the microcalli actively proliferated into friable watery callus when they were subcultured on MS medium supplemented with 0.3 mg/l 2,4-D and 1.0 mg/l kinetin. Although the plant regeneration from the protoplasts-derived calli has not yet been obtained, the reproducible developmental step from protoplasts to callus in this study may facilitate the establishment of somatic hybridization using C. cristata as one parent.
OPTIMASI ANALISIS AMILASE DAN GLUKANASE YANG DIEKSTRAK DARI MISELIUM Pleurotus ostreatus DENGAN ASAM 3,5 DINITROSALISILAT Maman Rahmansyah; I Made Sudiana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/406

Abstract

Enzymatic activities of amylase (1.4-α-D-glucan glucano hydrolase) and glucanase (1.4-β-glucanase) that extracted from Pleurotus ostreatus mycelium determined through the reducing sugar accumulation. The enzymes assayed in amorphous cellulose (carboxymethylcellulose = CMC) and starch substrate, respectively. Complexity reaction of the sugar to DNS (3.5-dinitrosalicylic acid) become sensitive caused of adding up oxidative agent of sodium sulfite and phenol. Variation in substrate concentration, combine with incubation period and its temperature was able to promote optimum activities of amylase enzymes. The best possible activity of amylase was turn out on 25° C temperature incubation along 20 minutes in 5% starch substrate assessment. Incubation period more then 60 minutes on amylase and cellulase caused those activities leaved to 40 and 70% down, correspondingly. Modified DNS fastened on reducing sugar twice effectively compared to unmodified one.
VESICULAR-ARBUSCULAR MYCORRHIZAL STATUS OF PLANT SPECIES IN THE PEAT SWAMP FOREST OF SETIA ALAM JAYA, SEBANGAU, CENTRAL KALIMANTAN Suciatmih Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/407

Abstract

In order to describe the vesicular-arbuscular mycorrhizal (VAM) status of plants growing on peat soil, a study was carried out in the peat swamp forest of Setia Alam Jaya in Sebangau, Central Kalimantan. Out of 146 plant root samples belonging to 48 plant species from 25 families examined, all plants colonized by VAM fungi namely 14 (29.2%) high level, 32 (66.7%) medium level, and 2 (4.1%) low level respectively.
CONTROL REGION-mtDNA HETEROGENEITY OF KALIMANTAN FALSE GHARIAL (Tomistoma schlegelii) POPULATION: A PRELIMINARY STUDY Hellen Kurniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/408

Abstract

The preliminary genetic study on Kalimantan "false gharial" from the wild was reported. Eleven tail scutes were collected from eleven individuals that originally consisting of two individuals from Kapuas River, one individual from Sentarum Lake, Jelai River, Mapam River, Perian Lake, and Lamandau River, two individuals from Barito River and three individuals from Mahakam River. PCR amplifying and sequencing 451 nucleotides in average that can be aligned at the same length of control region mitochondrial DNA. Among 11 individuals found eight polymorphic sites that consisted four haplotypes (A, B, C, and D) respectively, which is haplotype A is dominant. Based on phylogenetic tree that constructed by Tamura-Nei parameter, false gharial population in Kalimantan can be divided into two population groups; there were Central-Eastern Kalimantan population group and Western Kalimantan population group. Based on the hypothesis of landmasses separating between central-eastern Kalimantan and western Kalimantan that known as Schwaner Mountains, the genetic distance D = 1.53% was expected to be equal to 20 million years.
KARAKTERISTIK BIODEGRADASI ALKIL SULFONAT LINEAR OLEH Pseudomonas aeruginosa I Made Sudiana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/409

Abstract

Detergent contained of Linear Alkyl Sulfonate (LAS) is toxic material to human, animal and microorganism. Strain S1 isolated from detergent contaminated soil was able to grow in media with LAS as a sole carbon source. LAS degradation took place under aerobic condition, with μmax of 0.31-h, Ks = 7.75 mg/L, Vmax = 1.04 mg/L.hour-1and Km = 8.119 mg/L. Analyses of 16s rDNA revealed that S1 is belonging to Pseudomonas aeruginosa.
THE EVIDENCE OF BACILLIFORM VIRUS A CAUSATIVE AGENT OF WHITE SPOT SYNDROME OF WHITE SHRIMP Penaeus merguiensis Hari Suprapto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/410

Abstract

The rod-shape virus particles were found in the hyperthrophied nucleus and cytoplasm of diseased white shrimp Penaeus merguiensis naturally infected by White spot syndrome (WSBV). In natural infection cumulative mortality of shrimp were very high, 95% of population were dead in 3-7 days, and 5% of shrimp population survived. The disease was outbreak in intensive culture farms used the closed circulation sea water system. Others crustacean such as crabs and bentatos were not infected by WSBV at time of sampling. The virus particle were rod-shape ranging from 78 ± 10 nm in diameter and 280 ± 10 nm in length. The pathogenic bacteria mainly dominated by genus Vibrio sp were isolated from shrimp.
PENGGUNAAN ZOLETIL DAN KETAMINE UNTUK ANESTESIA PADA FELIDAE I Komang Wiarsa Sardjana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/411

Abstract

Zoletil and ketamine as a non barbiturat anaesthetic can be administered by the intramuscular route in Felidae a specially in the wild animals and pets. Seven Felidaeof the wild animals there were five the Lions (Panthera leo) and two the White tigers (Panthera tigris tigris) of the Surabaya Zoological Garden have of Zoletil with dose 5 mg/kg body weight and seven Cats of Veterinary Hospital of Faculty of Veterinary Medicine of airlangga was used Ketamine as anaesthetic with dose 20 mg/Kg body weight. All the animals have injected Atropin sulfate with dose 0.1 mg/Kg body weight intramuscular as a premedication. The result of this study of Zoletil in Felidae are shown that the animals have not in respiratory depression and during anaesthesia have done the body temperature means about 36.9º C, Pulsus rate is 100.8 times/minutes and Respiration rate is 21.7times/minutes. The studi of Ketamine the data shown during anaesthesia the means of the body temperature of the cats is 38.4º C with pulsus rate is 85.1 times/minutes and respiration rate is 41.1 time/minutes. We have assumed that study of the drug have a great effect of the animals in practice look like in cats or the wild animals for restraint or anaesthesia of short duration.
PENGARUH EDTA DALAM MENGHILANGKAN KADAR KADMIUM YANG TERAKUMULASI DALAM BERBAGAI ORGAN UDANG Macrobrachium sintangense de Man Agoes Soegianto; M. Yusuf Alamudi; Miftakhul Ulum; Eko W. Putro; Nining D. Widyayanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/412

Abstract

The objective of experiment is to know capability of EDTA to reduce the concentration of cadmium, which accumulated in Macrobrachium sintangense organ. Two groups of shrimps were exposed to 30 mg/L and to freshwater (control) during 7 days and continue with EDTA 0.1 and 1 ppm during 7 days. After finish the experiment, gill, hepatopancreas, carapace and abdominal muscle dissected and destructed with concentrated HNO3 to be analyzed the concentration of cadmium which is accumulated in those organs. The result showed that the concentration of cadmium in gill of control shrimp are 1.696 ± 0.3920 mg/Kg, hepatopancreas 1.1810 ± 0.545 mg/Kg, carapac 0.1025 ± 0.1524 mg/Kg, and abdomen 0.201 ± 0.349 mg/Kg. The concentration of cadmium in exposed shrimps which treated with 0.1 ppm of EDTA are 66.4090 ± 3.22 mg/Kg in gill, 26.4791 ± 0.86 mg/Kg in hepatopancreas, 8.8039 ± 0.66 mg/Kg in carapac; and 3.6324 ± 0.13 mg/Kg in abdomen. The concentration of cadmium in exposed shrimps which treated with 1 ppm of EDTA are 34.8809 ± 4.24 mg/Kg in gills, 9.7290 ± 1.03 mg/Kg in hepatopancreas; 4.6574 ± 0.34 mg/Kg in carapac; and 1.8546 ± 0.43 mg/Kg in abdomen. The greater elimination of cadmium occurs at the shrimp, which is treated with 1 ppm of EDTA.
PROFIL HUTAN MANGROVE TAMAN NASIONAL BALURAN JAWA TIMUR (Mangrove Forest Profile of Baluran National Park East Java) Sudarmadji Sudarmadji
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/413

Abstract

Five mangrove profiles at Baluran National Park East Java were studied. The location was Popongan, Batu Sampan, Kelor-Manting, Si Rondo Si Macan, dan Bilik beach. Each profile was found to be different in their distribution and population. They have demonstrated that there is no constant sequence for mangrove of contrasted areas, because some species tend to prefer a more seaward of a more landward site.
AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN "HOLE-PLATE DIFFUSION METHOD" Farida Lanawati Darsono; Stephanie Devi Artemisia
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 1 (2003): December 2003
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/414

Abstract

A study has been performed on the antimicrobial activities of “jambu biji” (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of “jambu biji” are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of “jambu biji” for each cultivar were obtained by reflux with ethanol 96%. The concentrations of the extracts applied to the holes were 10%, 20%, and 30% w/v, the extracts were reconstituted with tween 80 and ethanol 96%. Based on the result of the study, it can be concluded that the extract of “jambu biji” from each cultivar with the concentration of 10%, 20%, and 30% w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3×3 and furthery tested for significancy (α = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.

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